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10.3324/haematol.2010.030759 85

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Half-life ~30 min plasma half-life reported in rat models (subcutaneous administration)

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Several factors influence the choice of needle and syringe: Type of injection : Intramuscular (IM) or subcutaneous (SC) Gauge of the needle : Diameter of the needle Length of the needle : Determines the depth of injection Volume of the syringe : Determines the amount of B12 to be injected 3

Common approaches include: 10% acetic acid in water 0.1% trifluoroacetic acid (TFA) 0.1% formic acid After initial dissolution, the acidified solution can often be diluted with plain water or buffer